Molecular Biology Grade Agarose

Due to its low EEO (0.12), DNA sized between 100 - 50,000bp can be efficiently separated. A high gel strength of >1,300g/cm2 (1% gel) allows the separation of larger DNA molecules.

Recommended for Southern and Northern blotting applications where high purity and low sulphate is required. The gel also exhibits low background fluorescene after ethidium bromide staining.

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High Resolution Agarose

Most suited for the resolution of fragments between 250 and 1,000 base pairs. Varying the concentration of the gel allows effective resolution of different sized products.

To achieve the best resolution with high resolution grade agarose gels, the gels must be chilled at 4 - 8oC for 30 minutes prior to use. This is important in these low gelling temperature agaroses as the gelling process may not gel to completion at room temperature resulting in weak gels and poor resolution. The gel exhibits low background fluorescence after staining with ethidium bromide.

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Ultra High Resolution Agarose

Most suited for the resolution of fragments of up to 500 base pairs. Varying the concentration of the gel allows effective resolution of different sized products.

To achieve the best resolution with ultra high resolution grade agarose gels, the gels must be chilled at 4 - 8oC for 20 hours prior to use. This is important in these low gelling temperature agaroses as the gelling process may not gel to completion at room temperature resulting in weak gels and poor resolution. The gel exhibits low background fluorescence after staining with ethidium bromide.

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 Low Melting Point Agarose

Most suited for extraction and purification of nucleic acids from agarose gel. This is due to the low gel strength (400gms/cm2 at 1%) and the low melting temperature (26.9oC) properties of this gel. DNA products of >1,000 bp can be finely resolved with this gel.
To achieve the best resolution with low melting point grade agarose gels, the gels must be chilled at 4 - 8oC for 20 hours prior to use. This is important in these low gelling temperature agaroses as the gelling process may not gel to completion at room temperature resulting in weak gels and poor resolution. The gel exhibits low background fluorescence after staining with ethidium bromide.

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 Potato starch, hydrolysed

This high purity starch has been specifically prepared and tested for starch gel electrophoresis applications. Suitable for applications such as serum protein analysis and isozyme analysis.

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10x TBE (Tris-Borate-EDTA)

Each pack contains 4 sachets of buffer mix. Each sachet is sufficient to make 1 litre of 10x buffer.

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10x Tris- Glycine (Laemmli Buffer)

Each pack contains 4 sachets of buffer mix. Each sachet is sufficient to make 1 litre of 10x buffer.

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10x PBS (Phosphate Buffered Saline)

This high purity starch has been specifically prepared and tested for starch gel electrophoresis applications. Suitable for applications such as serum protein analysis and isozyme analysis.

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2x Gel Loading Buffer

Fisher Biotec 2x Gel Loading Buffer icontains 5% Ficoll 400, 0.8% bromophenol blue, and 0.8% xylene cyanol in TE Buffer pH 8.0.  The bromophenol blue and xylene cyanol dyes are used for tracking migration during electrophoresis.  In 0.5 - 1.2% agarose gels using 0.5x TBE bromophenol blue migrates at approximately 300bp and xylene cyanol at approximately 4kb.

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6x Gel Loading Buffer

Fisher Biotec 6x Gel Loading Buffer contains 15% Ficoll 400, 0.25% bromophenol blue, and 0.25% xylene cyanol in TE Buffer pH 8.0.  The bromophenol blue and xylene cyanol dyes are used for tracking migration during electrophoresis.  In 0.5 - 1.2% agarose gels using 0.5x TBE bromophenol blue migrates at approximately 300bp and xylene cyanol at approximately 4kb.

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